C5 Antikörper

Produktnummer ABIN111066

Dieser Anti-C5 Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von C5 in WB, IHC (fro) und EIA. Geeignet für Human, Schwein, Rind (Kuh), Ziege und Primate.

Kurzübersicht für C5 Antikörper (ABIN111066)

Target: C5 (Complement Component 5 (C5))

Reaktivität: Human, Schwein, Rind (Kuh), Ziege, Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser C5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: HCC5b-1 (neo)

Produktdetails anti-C5 Antikörper

Spezifität: HCC 5b.1 (neo) allows detection of C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis. Does not react with native C5! Polypeptide Reacting: Mr 200 000 polypeptide of SDS denaturated complement component C5 and C5b of Mr 185 000, after C5b activation, an Mr 60 000 cleavage product also reacts (weakly). Disorders Specifically Detected: Detection of C5b-9 complexes via activated C5, whereas native C5 reacts with antibody HCC 5.1.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Primates, Bovine, Pig, Goat.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Activated Human Complement Component C5

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Immunoblotting (Western blot). Cytological material. Immunohistochemistry on Frozen Sections (1/10). Incubation time: 1h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL distilled water.

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Details zu C5

Target: C5 (Complement Component 5 (C5))

Andere Bezeichnung: Complement C5

Hintergrund: C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190 kDa glycoprotein consisting of a disulphide linked alpha-chain (111 kDa) and beta-chain (75 kDa). C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).Synonyms: CPAMD4, Complement Component 5

Gen-ID: 727

NCBI Accession: NP_001726

UniProt: P01031

Pathways: Komplementsystem, Carbohydrate Homeostasis